The Vitamin D kit is a rapid, immune-chromatography based one step test. It is designed for Semi-qualitative determination of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human blood. This assay provides a preliminary diagnostic test result and can be used to screening for Vitamin D deficiency. The liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays or other quantitative immunoassays are recommended to further confirm the diagnostic test results